CRISPR Seed Resurrection is a project that will use CRISPR CAS 9 techniques to encode and change plants and their resultant seeds, to be more immune to higher rates of C02 in the atmosphere and to extend their ability to remain viable as seeds longer.

As pollen and seeds are reproductive adaptations, they did not appear in plants until millions of years after seedless plants had already begun to live on land.

These CRISPR CAS 9 seeds will be techno-augmented to be able to sprout in a distant future, genetically modified to allow them to survive in a future where global warming has harmed plants, not able to adapt quickly enough to our increasing C02 in the atmosphere. While most plant species reproduce with seeds, many species of seedless plants have become extinct, although seeds can also resurrect plants, long thought to be extinct.

The durability of seeds is known to depend critically on how it is stored, and if you keep it in cold, dry conditions, it can be kept alive for longer.

In 2006 a story from Israel had scientists grow a date palm from a 2,000-year-old seed, found during an archeological excavation on Mount Masada. The date palm seed was verified by radiocarbon dating.

In 2010 a Russian team found a seed cache of Silene stenophylla at 124 feet (38 meters) below the permafrost. stenophylla is a flowering plant native to Siberia, that had been buried by an Ice Age squirrel near the banks of the Kolyma River and radiocarbon dating confirmed that the seeds were 32,000 years old.

In 2018 a study in the Journal of Ecology reported that Mallards pick up seeds while feeding in the water and on land. In a process called ‘endozoochory,’ some of these seeds are not digested and survive gut passage intact and allow them to sprout after passage. It is now realized that Mallards and other waterfowl a significant vectors of moving seeds from lakes to lakes.

In this project, some seeds will be designed for dispersal by water foul with nutritious special coatings that will be developed based on the nature of seeds, and how they can naturally withstand the digestive systems of birds.

Other seeds will be designed and placed in metal seed pods specially designed to open at intervals of 1,000, 2,000, 3,000, and 10,000 years.

Metals that will degrade will be used to allow the pods to dissolve once they are exposed to the atmosphere as global warming continues and melts the snows.

My project will fly these metal seed pods in planes to a height, that will allow them to fall to the ground and melt into the ice and permafrost.  As they fall heat generated during their fall with the fins will enable them to melt into the snow and glaciers.

As the snow and glaciers melt, the metal will degrade and enable pods with Crispr Cas 9 seeds and pollen within to propagate new plants and start to repair the atmosphere with fresh oxygen being produced.

 

I am looking at many kinds of plants and at the moment focused on Arabidopsis given we have a large Arabidopsis seed bank at The Ohio State University.

Targeted genome editing using artificial nucleases may have the potential to allow plant breeding by providing the means to modify the genomes rapidly in a precise way using CRISPR Cas9.

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) systems, have been developed allowing site-specific double-stranded DNA breaks. While there are strengths and weaknesses of this technology as opposed to well-established genome editing such as using zinc-finger nucleases (ZFNs) as well as transcription activator-like effector nucleases (TALENs) recent research on plants using CRISPR/Cas9 technology, may allow future developments in this area, of directed editing to allow the plant to be optimized to global warming in the future.

As genetically modified seeds must undergo stringent testing and implementation in controlled environments, for exhibitions the pods and the seeds will be presented physically as rapid-prototyped samples. High res printed visualizations as above would also be a part of any exhibition.

All the research and CRISPR Cas 9 modifications will be done and planted, in a controlled environment for testing inside, in a variety of atmospheric conditions to allow the plant to express its new gene modifications, in an increased C02 environment.

 

Created by Ken Rinaldo; Concept, 3D modeling, and direction

Danner Seyfer Sprague; 3D modeling

TradeMark Gunderson; rapid prototyping and construction assistant